ACUTE PER ORAL TOXICITY OF TILMICOSIN – SUBSTANCE "BIOVET" AD IN WHITE RATS (FISCHER – 344)

Abstract

The acute oral toxicity of Tilmicosin – a substance "Biovet" in rats, was studied. 42 male white rats, Fischer 344 line, weighing 160–180 g, were treated with 10% aqueous solution of Tilmicosin phosphate Biovet using a gastric tube. For each dose (500, 1000, 1500, 2000, 2500, 3000 and 3500 mg/body weight), 6 were treated once. Rats, which were monitored for 7 days, taking into account the clinical picture of their intoxication and mortality. Based on the Litchfield-Wilcoxon method used in the test, an average lethal dose of LD50 2200 (2860 ÷ 1692) mg/body weight (p = 0.05) and relatively lethal doses of LD16 = 1142 and LD84 = 3256 mg/body weight were determined, which classify the product as low toxic. The results regarding the acute toxicity of Tilmicosin when administered orally to white rats confirm the studies conducted with the original Eli Lilly product that Tilmicosin has low toxicity when administered for oral use – LD50 > 2000 mg / body weight (Piroozi, K.S. et al. 1993).