vol.2. N 1. 2017
BIOCHEMICAL STUDIES OF RATS AFTER BONE IMPLANTATIONS OFMATERIALS ON THE BASE OF TTCP/ DCPA, TARTARIC OR ASCORBICACIDS
M. Gabrashanska, S. Tepavitcharova, R. Ilieva, M. Alexandrov, V. Nanev,N. Tsocheva-Gaytandzhieva, I. Vladov, P. Dimitrov
The study aimed to evaluate hematological levels of markers of bone turnover in rats with calvarialdefects filled with modified calcium phosphates cements. Cements were synthesized basing on TTCP/DCPA,carboxylic acids, xanthan gum and glycerin. Total and bone alkaline phosphates, osteocalcin, and their relationwith oxidative stress (malondialdehyde) were studied. No differences in the markers levels were observedbetween the groups with implants. The level of free radicals was significantly increased in all groups. Theobtained data might be useful in future experiments with new bone implants.
Key words: cements, bone markers, oxidative stress
TMVM, 2017, vol.2, No 1(1): 3–9; ISSN 2534-9333
CONTINUED RESEARCH TO ESTABLISH THE CRITERION FOR ASSESSING THE QUALITY OF EDUCATION IN VETERINARY MEDICINE
The quality of education is a problem that exists both in primary and secondary and higher education.Way to illustrate the utilization of relevant knowledge and skills by students remains evaluation system. By analyzing the results of the average grade of semester exams and that of final exams in graduating of the students of Faculty of Veterinary Medicine at the University of Forestry by the years for 20 years (1995–2015) and a total of 16 graduates, authors (Petrova, Kostadinov) aim to promote criterion assess the quality of training in order of following differences between the values of the average of semester exams and average of final examinations in graduation: 0.5 to 1.0 and 1.0 above.
Key words: education, average of semester exams, average of final examinations in graduation, examination commissions, veterinary medicine, quality of education
TMVM, 2017, vol.2, No 1(1): 9–12; ISSN 2534-9333
COMPARATIVE ECHOCARDIOGRAPHIC EXAMINATIONS IN DOGS WITHMYXOMATOUS DEGENERATION OF THE MITRAL VALVE DEPENDINGON THE PRESENCE OR ABSENCE OF PULMONARY EDEMA
Atanas Pankov, Hristina Shukerova, Geno Angelov
In the article echocardiographic studies of 12 dogs with myxomatous degeneration of the mitral valvewere compared. Patients were divided into two groups according to the presence or absence of pulmonaryedema. The echocardiographic measurements were indexed to Ao – size. We received significant difference(p = 0.0077) only for LA / Ao, but this ratio can not be a predicator for the development of heart failure.
Key words: dogs, myxomatosis, mitral valve, pulmonary edema
TMVM, 2017, vol.2, No 1(1): 13–16; ISSN 2534-9333
BONE REGENERATION IN CRITICAL-SIZE CALVARIAL DEFECT IN RATS USING INNOVATIVE NANO COMPOSITE MATERIAL OF CHITOSAN / NANOHYDROXYAPATITE COMPOSITE
Petar Dimitrov, Elena Dyulgerova, Radost Ilieva, Radosveta Vasileva,Veselin Nanev, Ivelin Vladov, Margarita Gabrashanska, Marin Alexandrov, Neli Tsocheva-Gaytandzhieva
Healing of standardized critical-size calvarial defects in rats was performed with nano composite material of nano-hydroxyapatite/3 % solution of chitosan in citric acid, and electrospinning fibres of poly lactic acid. Histologically at the 84 day, the implanted rats exhibited full closure of the defect by new developed connective and osteoid tissues penetrating in reticular pattern within the implant components. These results indicate that by using a newly created composite paste critical cranial defects can be successfully retrieved and probably is also suitable for other cases in bone augmentation surgeries.
Key words: calvarial defect in rats, chitosan/nano-hydroxyapatite, bone regeneration
TMVM, 2017, vol.2, No 1(1): 17–20; ISSN 2534-9333
OSTEOCHONDRODYSPLASIA IN SCOTTISH FOLD CATS CASE REPORT
Nadya Zlateva, Georgi Marinov
The Scottish Fold breed of cat has a genetic mutation that affects the development of cartilage. The most obvious manifestation of this is typical appearance of the ears of Scottish Fold cat. This genetic defect also causes abnormalities of the cartilage of bones. Scottish Fold osteochondrodysplasia (SFOCD) is characterized by skeletal deformities such as short, thick, and inflexible tails and shortened splayed feet. Two Scottish Fold cats aged between 8 months and 1 year were presented for signs of skeletal disease including lameness, pain and reluctance to jump. A diagnosis of osteochondrodysplasia was based on typical characteristic radiological findings. Clinical signs were ameliorated by administration of non-steroidal anti-inflammatory drugs and drugs, contain glycosaminoglycans. Definitive treatment of the disease is impossible. Survival of the cats varies depending on the severity of clinical signs.
Key words: osteochondrodysplasia, Scottish Fold cat
TMVM, 2017, vol.2, No 1(1): 21–24; ISSN 2534-9333
OLEUM OREGANO – PROPERTIES AND APPLICATION
Тhe tendency seems to be search of natural and ecological products for application in the veterinary medicine. Biological active substances of plant origin are such group. One of the example is oregano essential oil. It has an application in veterinary medicine. It essential compounds are: timol, carvacrol, γ-terpinen, pcimen, terpineol, trans-sabinen-hydratum. A number of properties have been proven antimicrobial, antifungal, nutritional, antioxidant, spasmolytic, cytostatic, antiparasitic and antihepatotoxic of oregano essential oil. The aim of this reviw is to present new data about its theraupeutic application.
Key words: oregano essential oil, compounds, properties, new data, application.
TMVM, 2017, vol.2, No 1(1): 25–30; ISSN 2534-9333
INVESTIGATION ON MALIGNANT CATARRHAL FEVER VIRUS IN WILD RUMINANTS
Martin Hristov, Reneta Petrova, Marin Alexandrov, Raiko Peshev
Malignant catarrhal fever (MCF) is a fatal disease responsible for the mortality of domestic, wild ruminant species and those inhabiting zoological garden. The aim of this study is to investigate pathohystological and molecular biological peculiarities of MCF viruses isolated in large and small wild ruminants from the zoos of Sofia and Varna. Different samples originated from 14 dead and alive wild animals (4 bisons, 3 gaurs, 2 yaks, camel, 2 cameroon goats, 1 hippopotamus, and 1 capricorn) were used for investigations. Typical pathological changes have observed as mild circumferential limbal keratitis, disseminated foci of consolidation of pulmonary lobes, hemorrhagic cystitis, superficial necroses in small intestine and petehiae and haemorrhages in fore stomach. Bronchointerstitial pneumonia with hyaline membrane formation, lymphocytic-histiocytic endarteritis and phlebitis, hemorrhages in urinary bladder, hypoplasia of spleen, necrosis of the superficial epithelium of intestine and ulceration, in lymph node subcapsular lymphoid and histiocytic trafficking, neutrophils and mononuclear cells and carbonate uroliths in kidneys, subendocardial and subepicardial haemorrhages and huge necrosis in hard musculature were observed. By conventional and nested polymerase chain reaction (PCR) for MCF virus of organ samples and cell cultural MCF virus isolates bands with molecular weight of 422 bp and 238 bp respectively were observed.
Key words: malignant catarrhal fever, pathohistological signs, PCR and wild ruminants.
TMVM, 2017, vol.2, No 1(1): 31–38; ISSN 2534-9333
ENTEROBACTER AGGLOMERANS – A CAUSE OF STOMATITIS IN A SNAKE
Seven Mustafa, Teodora Popova
Microbiological investigations were carried out of material from the mouth of a common boa (Boa constrictor imperator) with signs of stomatitis, general weakening of the body, difficulty eating and breathing. In the result of the microbiological examinations of the sample from the lesions in the oral cavity were isolated Enterobacter agglomerans 5 and Aspergillus sp. Successful therapy was carried out with enrofloxacin, selected according to the results of the antibiotic gram. The dosage administered was 5 mg/kg Baytril 5 % daily by intramuscularly injection for 10 days. The local lesions were treated with a silver sulfadiazine – cream 1 % and clotrimazole – cream 1 % once a day for 3 weeks up to the recovery.
Key words: stomatitis, Boa constrictor imperator, Enterobacter agglomerans, Aspergillus sp., therapy.
TMVM, 2017, vol.2, No 1(1): 39–44; ISSN 2534-9333
GENETIC PREDISPOSITION TO DISEASES OF THE BREED CZECHOSLOVAKIAN WOLFDOG
K. Karneva, M. Stefanova-Georgieva, A. Korniotis, J. Mihaylova, G. I. Georgiev
Czechoslovakian wolfdog (CHSV) is a quite new dog breed created and raised for military purposes. In 1955 a biological experiment has been made in Czechoslovakia for obtaining a litter between a German Shepherd dog (NOC) and a Carpathian wolf in order to select the most useful qualities of the two. In 1982 the standard for the breed Czechoslovakian wolfdog has been approved and from 1999 it is acknowledged from the International Cynological Federation (FCI). Quite recently – 2010, the first representatives have been imported in Bulgaria. With the increasing worldwide interest and number of puppies emerge questions regarding genetic predispositions to diseases and indispensable veterinary healthcare. Despite the fact that it is a young and quite healthy breed we have focused on characteristic traits such as susceptibility to hip and elbow dysplasia, de-generative myelopathy, pituitary dwarfism and gastrointestinal diseases related to gene inheritance within the wolf and the German shepherd. There has been an extensive research conducted including leading breeding farms, veterinary clinics and laboratories regarding the most frequently occurring problems, specificities and maladies. The information is extracted through clinical, radiological, endocrinological and polymerase chain reaction methods. The aim of this report is to acquaint owners with the nature of the breed upon choosing a pet; veterinary doctors – with the inherit predispositions of Czechoslovakian wolfdog, diagnostic possibilities and prevention.
Key words: Czechoslovakian wolfdog, Inherited diseases, Genetic research, Breeding, Prevention.
TMVM, 2017, vol.2, No 1(1): 45–52; ISSN 2534-9333
OFFER AND DEMAND OF GOAT’S MILK PRODUCTS IN PELAGONIA REGION R. MACEDONIA
Biljana Petrovska, Natasha Petrovska, Zhivko Gacovski, Goce Cilev, Nikola Pacinovski, Igor Zdraveski, Zlatko Dimeski
Goat breeding in the Republic of Macedonia is a sector of husbandry industry that has a good basis for improving the genetic capacity of livestock breeding, increasing the volume of production and creating opportunity for economic profit of the farmers. Considering the climatic conditions and the experiences of other countries, the Republic of Macedonia has capacity for adequate development of goat breeding by establishing smal to medium goat farms and application of intensive or semi-intensive goat farming systems. There are various breeds of goats in the Republic of Macedonia such as the Domestic Balcan Goat, Saanen and Alpina and cross-breeds between these breeds. The basic product that is obtained from goats is goat milk which is mostly processed into cheese, butter and yogurt. Subject of research in this paper is the supply and demand of products from goat’s milk in the Pelagonia region. The research includes data on supply of products from goat’s milk taken from the Ministry of Agriculture, Forestry and Water Economy of the Republic Macedonia and data on demand of the products obtained using a questionnaire on a representative sample of 200 respondents chosen at random. The largest percentage (70 %) of the respondents said that the market offers small quantities of products from goat milk. Therefore, it is nessery to take more measurements aiming the development of this industry in the future.
Key words: Goat Breeding, Supply, Demand, Products.
VECTOR BORN DISEASES IN DOGS – DIROFILARIOSIS AND ANAPLASMOSIS. A CLINICAL STUDY
Boris Borisov, Georgi Marinov, Panayot Panayotov, Nadya Zlateva
Canine vector-borne diseases are an important group of illnesses affecting dogs around the world. The transmission of these diseases to the dogs is through different arthropod vectors, such as ticks, fleas, lice, triatomines, mosquitoes, tabanids, and phlebotomine sand flies. For the period of years 2014–2016 we made 160 tests for these diseases. Twelve of these animals were positive for Dirofilaria immitis, 14 were positive for Anaplasmosis and 10 of them were positive for Ehrlichiosis. There were no positive tests for Lime disease. Part of these animals was treated through a specific therapy according to their condition.
Key words: vector-born diseases, dog, dirofilariosis, anaplasmosis
CHEMICAL ELEMENTS IN FASCIOLA HEPATICA (TREMATODA) AFTER DIETHYLNITROSAMINE TREATMENT
Veselin Nanev, Margarita Gabrashanska, Neli Tsocheva-Gaytandzhieva
The aim of the work was to be investigated the trace elements content in Fasciola hepatica under the toxic effect of diethylnitrosamine (DENA). The study of the trace elements in the injured from DENA helminthes showed specifically changed picture. The levels of Zn and Fe were heavily decreased but Rb – slightly reduced. The contents of Cu, Co, Br, Mo and Se were similar to those of the intact helminthes. The content of Cr was increased. We supposed that the morphological changes of F. hepatica on the background of DENA intoxication lead to a mineral imbalance related to the pathophysiology of the helminthes.
Key words: Helminthes tissues, chemical intoxication, trace elements.
QUALITY AND HYGIENIC CORRECTNESS OF GOAT MILK IN THE SKOPJE REGION OF R. MACEDONIA
Goce Cilev, Nikola Pacinovski, Zhivko Gacovski, Biljana Petrovska, Tamara Isjanovska, Zlatko Dimeski
The aim of this experiment was examine the quality and hygienic correctness of goat milk. The researches were conducted in the producing conditions in at 3 goats farm “Taor”, “Kozhle” and “Ajvatovci” the villages near by the Skopje, R. Macedonia. The experiment is carried out on goats in lactation period with 9 milk controls per each farm, in period April-total 27 samples and the same situation in May 2015 year. Average Milk Fat Content (AMFC) and Average Milk Protein Content (AMPC) in the three goat farms in month April/2015 were 3.68 % and 3.34 respectively, but AMFC and AMPC in the three goat farm in month May/2015 were 3.74 % and 3.23% respectively. Average Somatic Cells Content (ASCC) in the three goat farm in month April/2015 were 924.667/ml milk and Average Somatic Cells Content (ASCC) in the three goat farm in month May/2015 were 518.667/ml milk. Average Bacteria Content (ABC) in the three goat farm in month April/2015 were 1.688.667/ml milk, Average Bacteria Content (ABC) in the three goat farm in month May/2015 were 1.517.333/ml milk. About chemical composition of goat milk in Skopje region is in correlation of article 7 from Regulation for quality of raw milk (Gazette of RM No. 96/11), the same situation is about somatic cells in goat milk in Skopje region is in correlation of article 7 from Regulation for quality of raw milk (Gazette of RM No. 96/11). But the about total number of bacteria of goat milk in Skopje region is increase and not corresponding with Regulation for microbiological quality of raw milk and classified goat milk in the II class of quality. This work attemps to present the quality and hygienic correctness of goat milk in separate region of R. Macedonia and future measures to better quality of goat milk.
Key words: goat milk, quality, milk fat, milk protein, somatic cells, bacteria.